![](https://parts.igem.org/images/partbypart/icon_t7.png)
T7
Part:BBa_K3476011:Design
Designed by: Silvio Salvatore Bonni Group: iGEM20_MSP-Maastricht (2020-10-19)
bacterial shRNA_expression_cassette
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 56
Illegal NheI site found at 116
Illegal NotI site found at 31 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 56
Illegal BamHI site found at 62 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 56
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added T7 terminator from T444T for effective transcription termination.
Source
Plasmids: L4440; T444T